Isolation and Characterization of Chemical Compounds from the Stem Bark Fractions of Citrus Grandis and Evaluation of Their Antimicrobial, Cytotoxic, and Antioxidant Activities

Citrus grandis belongs to the Rutaceae family and is a naturally occurring citrus plant. The fractionated crude extracts of C. have been studied for investigation its phytochemicals allowed evaluation biological activities, especially antimicrobial, cytotoxic, antioxidant activities. At first, we assessed antibacterial antifungal properties using disc diffusion method conducted cytotoxicity screening brine shrimp lethality bioassay. Among raw extracts, isolated two compounds named CG-F3-B1 CG-F3-B2 through column chromatographic separation chloroform extract. By comparing their proton NMR spectra with published spectra, identified chemical structures these as Zeylenol Limonin, respectively. Regarding testing, observed that methanol soluble fractions petroleum ether exhibited mild moderate activity against bacteria. Additionally, ethyl acetate fraction showed activity. In addition, extract displayed specific fungi. Furthermore, cytotoxic assay revealed relevant effect an LC50 value 37 μg/ml. was determined DPPH assay, modest IC50 89 Our findings demonstrated stem bark from various promising including antibacterial, antifungal, properties.
 Bioresearch Commu. 9(2): 1320-1329, 2023 (July)